Single Cell MicroRNA Analysis Using Microfluidic Flow Cytometry
نویسندگان
چکیده
منابع مشابه
Single Cell MicroRNA Analysis Using Microfluidic Flow Cytometry
MicroRNAs (miRNAs) are non-coding small RNAs that have cell type and cell context-dependent expression and function. To study miRNAs at single-cell resolution, we have developed a novel microfluidic approach, where flow fluorescent in situ hybridization (flow-FISH) using locked-nucleic acid probes is combined with rolling circle amplification to detect the presence and localization of miRNA. Fu...
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ژورنال
عنوان ژورنال: PLoS ONE
سال: 2013
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0055044